tube medium Learn more about tube medium
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Technical regulation for production of edible bacteria
Edible strains refer to artificially cultured pure mycelium of edible fungi, which are usually divided into mother species, original species and cultivated species. Mother species are pure mycelia identified as valuable for production through strict screening. The mother seed is cultured with test tube slant medium, which is also called test tube species or first-class species. The mother seed is propagated and transferred to sawdust or grain culture medium to obtain the strain, which is called the original seed or secondary species. The culture container is mostly a can bottle or a strain bottle. The strain propagated from the original seed is called cultivated species or tertiary species, which can be directly used for production and cultivation. 1. Preparation of mother seed 1. Preparation of mother seed
2019-01-16 -
Production technology of Armillaria mellea and germinating bacteria of Gastrodia elata
Artificial cultivation of Gastrodia elata needs two kinds of symbiotic bacteria: germinating bacteria and Armillaria mellea in the process of sexual reproduction. In the production practice, the author found out the culture medium and production technology which can grow both of the two kinds of symbiotic bacteria. The specific practices are as follows. 1. Liquid culture medium: potato (sprouting, peeling, slicing) 100g, hard sawdust, wheat bran, corn flour 50g each, tap water or natural water 1300mL, boiled in a double pot for 30 minutes, then filtered with double gauze. Take filtrate to replenish water to 1000mL
2019-01-16 -
Seed Production Techniques of Auricularia auricula
Black fungus hanging bag cultivation 2-3 tide ear, due to the bag nutrition, lack of water no longer out of the ear, generally discarded waste material. In practice, we adopt sand coating treatment, after many tests can still produce 3-4 ears, the specific methods are as follows: take off the bacteria bag, bacteria material placed in nutrient solution soaked for a day, let it absorb enough water (nutrition).
2018-09-11 -
Main points of inoculation technology of edible fungi
Inoculation is one of the key links in the production of edible fungi. If the inoculation technology of female fungi is not up to standard, it will cause contamination of miscellaneous fungi, and the survival rate is low and all previous efforts are wasted, let alone cultivation. Whether it is strain isolation, passage or propagation, it must be carried out under aseptic conditions in strict accordance with aseptic operating procedures. The specific points are as follows: 1. Personnel quality 1.1 Vaccination personnel should have the spirit of seriousness, meticulous, self-consciousness and responsibility for the cause. 1.2 Inoculators should firmly establish the concept of sterility and should not be lucky.
2019-01-15 -
Practical techniques for seed production of Edible Fungi
Practical techniques for seed production of Edible Fungi
2019-08-02 -
Propagation technique of virus-free test-tube plantlets of potato
Potato is the fifth largest crop in Heilongjiang Province, only after rice, wheat, corn and soybeans. it can be used as food, vegetables, industrial raw materials and other crops. Potato is still a new industry in Heilongjiang Province, with a history of only more than 20 years. The formation and development of this industry is accompanied by the research and development of potato detoxification technology. Through the smooth implementation of the poverty alleviation and development project of "joint construction of courtyards and counties" and under the guidance of experts and professors of the Provincial Academy of Agricultural Sciences, Huachuan County has established Sujiadian Town and Lifeng Township.
2019-01-16 -
Practical seed production techniques and Identification of Edible Fungi Diseases
Practical seed production techniques of Edible Fungi
2019-06-03 -
Seven methods for the storage of edible fungi and notes on the introduction of edible fungi
Seven methods for Storage of Edible Fungi
2019-06-23 -
A simple method for making Mother species of Edible Fungi
PDA or PSA and their improved culture medium are mostly used in the production of edible mushroom mother seed, but in some remote areas or small batch production, the raw materials in the formula are difficult to be purchased in rural areas due to the high price (generally analytical purity) and delay production. This paper introduces several simple production methods. 1. Gelatin medium. Gelatin, which refers to edible gelatin, is sold in general food and chemical stores at a lower price. Gelatin used as a coagulant or solidifying agent in food production, instead of Agar (watercress, Agar) as coagulant in mother seed production.
2019-01-15 -
Seed production Technology of Edible Fungi
3. There are three methods for mother species separation: spore bullet firing, tissue isolation and matrix mycelium isolation. This paper mainly introduces the method of tissue separation. The method of tissue separation: small pieces of tissue from each mushroom were grafted on the bevel medium and cultured into the mother seed. The fruiting body of edible fungi is actually the knot of binuclear hyphae, which has a strong ability of regeneration. Therefore, as long as a small piece of tissue is cut, inoculated on a suitable medium and cultured at the right temperature, the pure mycelium can be obtained. Tissue separation is to bring the selected mushrooms into the sterile room, on the ultra-clean workbench, with 75.
2019-01-16 -
Preparation of Mother Culture medium for Edible Fungi
1. 800 grams of corn, 20 grams of glucose, 20 grams of Agar, 1.5 liters of rice water. First, boil the corn kernels in dilute Amoy rice water, pick them up when a small amount of corn kernels burst and filter. Continue to heat the filtrate (if the filtrate is less than 1 liter, add clean water to make up), add Agar and glucose, stir until all the Agar dissolves, then separate the test tube, sterilize and place the bevel. two。 Fresh Pleurotus ostreatus or mushroom foot 200 grams, glucose 20 grams, Agar 20 grams, water 1 liter. The preparation method is the same as potato glucose Agar medium. 3. Wheat bran 2
2019-01-15 -
Appearance Identification of Mother species of Edible Fungi
Excellent and purified strains are the fundamental guarantee for the successful cultivation of edible fungi. Several methods for identifying the mother species of edible fungi are introduced as follows: the mycelium of Lentinus edodes on potato glucose Agar (PDA) medium is pure white, stout, flocculent or fluffy, has a certain wall-climbing ability after full test tube, and the edge is irregular and wavy. At the temperature of about 24 ℃, the mycelium can cover the inclined surface of the test tube in 15 days. With the special fragrance of Lentinus edodes. The hyphae of Pleurotus ostreatus were white, dense and stout on PDA medium.
2019-01-15 -
Four Reform measures of planting Ganoderma lucidum in bags
First, the culture medium changed sawdust into wood blocks. Ganoderma lucidum has a tough texture and needs to absorb a lot of lignin and cellulose in the process of growth. Using sawdust or cotton shell as the culture medium, due to the loose texture, the lignin in the matrix is easy to be decomposed by hyphae. If the use of wood blocks with coarse sawdust as the medium, the same volume contains more lignin, more gaps between wood blocks, good ventilation performance, conducive to the growth of Ganoderma lucidum, so high yield and high quality. Second, change indoor cultivation to field soil mulching cultivation. When Ganoderma lucidum is planted in indoor bags, the surface of the fungus tube is easy to form a layer.
2019-01-15 -
Experimental study on Industrial Rapid Propagation of Potato Test-tube plantlets
For many years, due to the self-breeding and self-breeding of farmers, the yield of potato decreased year by year, the quality became worse, the shape of the product became worse and so on. It has been proved by scientists that virus infection and its accumulation in potato blocks are the main reasons for potato "degradation". In order to solve the problem of serious degradation and yield reduction of main potato varieties in our city, our institute took potato virus-free production as a key experimental subject, and carried out experimental research on the problems existing in virus-free production, so as to make use of resources and reduce production costs.
2019-01-16 -
Three measures to breed high quality Pleurotus ostreatus strains
Three measures to breed high quality Pleurotus ostreatus strains
2018-06-30 -
High-yield cultivation techniques of Ganoderma lucidum
High-yield cultivation techniques of Ganoderma lucidum
2018-06-29 -
Tianning 38 wheat variety tube medium
There are a variety of wheat varieties, and different wheat varieties also have their own unique characteristics. How about Tianning 38 wheat? Let's take a look at Tianning 38 wheat variety Nosuke. Variety brief introduction of Tianning 38 Wheat Variety from Tianning seed Industry Co., Ltd.
2020-11-09 Tianning wheat variety tube medium -
Storage method of edible fungus strain and mastery of topdressing method of edible fungus
Storage methods of Edible Fungi
2019-06-13 -
Three measures for breeding High quality Pleurotus ostreatus
Three measures for breeding High quality Pleurotus ostreatus
2018-07-01 -
Prevention and control of diseases and insect pests of edible fungi, storage of edible fungi
Storage of edible fungi
2019-06-03